辣椒果实辣椒素类物质生物合成分子遗传学研究进展
Advances in Molecular Genetics of Biosynthesis of Capsaicinoids in Capsicum Fruit
ES评分9.25
| DOI | 10.3969/j.issn.2095-7300.2024.02.014 |
| 刊名 |
Journal of Hunan Ecological Science
|
| 年,卷(期) | 2024, 11(2) |
| 作者 |
|
| 作者单位 |
云南农业大学园林园艺学院,云南省高校蔬菜种业工程研究中心,云南昆明 |
| 摘要 |
辣椒是我国栽培面积最大的蔬菜作物,辣椒果实因为富含辣椒素类物质而呈现辣味,而辣椒素类物质在制药、化妆品和农用工业等领域具有多种用途。辣椒素类物质是通过香草醛胺(衍生自苯丙氨酸)与支链脂肪酸(缬氨酸或亮氨酸前体)缩合而成的,它们通常积聚在辣椒果实的胎座组织中。辛辣度取决于辣椒的基因型,但也受外部条件的影响。近年来,分子生物学和生物技术的发展促进了辣椒素类物质生物合成基因的鉴定,为培育辣椒素类物质含量更高的辣椒新品种提供了机会。本文综述了辣椒素类物质生物合成的部位、合成途径、关键结构基因、QTL位点、关键转录因子,以及外源辣椒素和胁迫反应对辣椒素生物合成的影响等方面的研究进展,并对该领域的研究现状和前景进行了展望,以期为辣椒果实辣椒素类物质的生物合成与调控研究提供指导。
|
| Abstract |
Capsicum is the largest vegetable crop in China. Capsicum fruit is spicy because it is rich in capsaicinoids, which have many uses in the fields of pharmaceuticals, cosmetics and agricultural in- dustries. Capsaicin-like substances are synthesized by condensation of vanillal amine (derived from phen- ylalanine) with branched chain fatty acids (valine or leucine precursors), which usually accumulate in the placental tissue of capsicum fruit. The spiciness depends on the genotype of capsicum, but it is also influenced by external conditions. In recent years, the development of molecular biology and biotechnolo- gy has promoted the identification of capsaicin biosynthesis genes, which provides an opportunity to culti- vate new capsicum varieties with higher capsaicin content. In this paper, the current understanding of the biosynthetic pathway of capsaicin in capsicum fruit and the ways of further research are reviewed to pro- vide guidance for the biosynthesis and regulation of capsaicinoids in capsicum fruit.
|
| 关键词 |
辣椒;辣椒素类物质;生物合成;分子遗传学;研究进展
|
| KeyWord |
capsicum;capsaicinoids;biosynthesis;molecular genetics;advances
|
| 基金项目 | |
| 页码 | 111-120 |
[1] 邹学校,胡博文,熊程,等. 中国辣椒育种60 年回顾与展望
[J]. 园艺学报,2022,49(10):2099-2118.
[2] CARVALHO A V,DE ANDRADE MATTIETTO R,DE OL- IVEIRA RIOS A,et al. Bioactive compounds and antioxidant activity of pepper (Capsicum sp. ) genotypes
[J]. Journal of Food Science and Technology,2015,52(11):7457-7464.
[3] ANTONIO A S,WIEDEMANN L S M,VEIGA JUNIOR V F. The genus Capsicum:A phytochemical review of bioactive secondary metabolites
[ J]. RSC Advances,2018,8 (45): 25767-25784.
[4] BAENAS N,BELOVIC' M,ILIC N,et al. Industrial use of pepper ( Capsicum annum L. ) derived products:Techno- logical benefits and biological advantages
[J]. Food Chemis- try,2019,274:872-885.
[5] FAYOS O,OCHOA-ALEJO N,DE LA VEGA O M,et al. Assessment of capsaicinoid and capsinoid accumulation pat- terns during fruit development in three chili pepper genotypes (Capsicum spp. ) carrying Pun1 and pAMT alleles related to pungency
[J]. Journal of Agricultural and Food Chemistry,2019,67(44):12219-12227.
[6] BOSLAND P W,COON D,REEVES G. ‘trinidad Moruga scorpion’ pepper is the world’ s hottest measured Chile pepper at more than two million scoville heat units
[J]. Hort Technology,2012,22(4):534-538.
[7] BUCHANAN M. Some like it hot
[ J]. Nature Physics, 2020,16(1):112.
[8] DENG M H,WEN J F,ZHU H S,et al. The hottest pepper variety in China
[J]. Genetic Resources and Crop Evolution, 2009,56(5):605-608.
[9] 李海龙,张俊清,赖伟勇,等. 海南黄灯笼椒与不同品种辣椒的辣椒素含量测定
[J]. 中国野生植物资源,2012, 31(4):32-34.
[10] WAHYUNI Y,BALLESTER A R,SUDARMONOWATI E, et al. Secondary metabolites of Capsicum species and their importance in the human diet
[J]. Journal of Natural Prod- ucts,2013,76(4):783-793.
[11] AZA-GONZáLEZ C,NU'?EZ-PALENIUS H G,OCHOA- ALEJO N. Molecular biology of capsaicinoid biosynthesis in chili pepper (Capsicum spp. )
[J]. Plant Cell Reports, 2011,30(5):695-706.
[12] STEWART C,MAZOUREK M,STELLARI G M,et al. Ge- netic control of pungency in C. chinense via the Pun1 lo- cus
[J]. Journal of Experimental Botany,2007,58 (5): 979-991.
[13] CISNEROSPINEDA O,TORRESTAPIA L,GUTIERREZ- PACHECO L,et al. Capsaicinoids quantification in chili peppers cultivated in the state of Yucatan, Mexico
[ J]. Food Chemistry,2007,104(4):1755-1760.
[14] MAZOUREK M,PUJAR A,BOROVSKY Y,et al. A dy- namic interface for capsaicinoid systems biology
[J]. Plant Physiology,2009,150(4):1806-1821.
[15] BOSLAND P W,COON D,COOKE P H. Novel formation of ectopic (nonplacental) capsaicinoid secreting vesicles on fruit walls explains the morphological mechanism for super-hot Chile peppers
[ J ]. Journal of the American Society for Horticultural Science,2015,140(3):253-256.
[16] TANAKA Y,NAKASHIMA F,KIRII E,et al. Difference in capsaicinoid biosynthesis gene expression in the pericarp reveals elevation of capsaicinoid contents in chili peppers (Capsicum chinense )
[ J ]. Plant Cell Reports, 2017, 36(2):267-279.
[17] TANAKA Y,WATACHI M,NEMOTO W,et al. Capsaicinoid biosynthesis in the pericarp of chili pepper fruits is associ- ated with a placental septum-like transcriptome profile and tissue structure
[ J]. Plant Cell Reports,2021,40 (10): 1859-1874.
[18] SUGIYAMA R. Capsaicinoids production and accumulation in epidermal cells on the internal side of the fruit pericarp in ‘bhut jolokia’ (Capsicum chinense)
[J]. CYTOLOGIA, 2017,82(3):303-306.
[19] PARK M,LEE J H,HAN K,et al. A major QTL and can- didate genes for capsaicinoid biosynthesis in the pericarp of Capsicum chinense revealed using QTL-seq and RNA- seq
[J]. Theoretical and Applied Genetics,2019,132(2): 515-529.
[20] ZHU Z S,SUN B M,CAI W,et al. Natural variations in the MYB transcription factor MYB31 determine the evolution of extremely pungent peppers
[J]. The New Phytologist,2019, 223(2):922-938.
[21] STEWART JR. C,KANG B C,LIU K D,et al. The Pun1 gene for pungency in pepper encodes a putative acyltrans- ferase
[J]. The Plant Journal:for Cell and Molecular Biol- ogy,2005,42(5):675-688.
[22] 雷建军,朱张生,孙彬妹,等。辣椒素类物质生物合成及其分子生物学机理研究进展
[J]. 园艺学报,2018, 45(9):1739-1749.
[23] 张正海,毛胜利,王立浩,等. 辣椒的辣味遗传控制与辣椒素生物合成研究进展
[J]. 园艺学报,2014,41(9): 1821-1832.
[24] 张婧,颉建明,郁继华,等. 辣椒素类物质的生物合成影响因素及其生理功能研究进展
[J]. 园艺学报,2019, 46(9):1797-1812.
[25] TANAKA Y,HOSOKAWA M,MIWA T,et al. Newly mu- tated putative-aminotransferase in nonpungent pepper (Capsicum annuum) results in biosynthesis of capsinoids, capsaicinoid analogues
[ J]. Journal of Agricultural and Food Chemistry,2010,58(3):1761-1767.
[26] ZSIBORáS C,MáTICS R,HEGYI P,et al. Capsaicin and capsiate could be appropriate agents for treatment of obesi- ty:A meta-analysis of human studies
[J]. Critical Reviews in Food Science and Nutrition,2018,58(9):1419-1427.
[27] UARROTA V G,MARASCHIN M,DE BAIRROS ? F M, et al. Factors affecting the capsaicinoid profile of hot pep- pers and biological activity of their non-pungent analogs ( capsinoids ) present in sweet peppers
[ J ]. Critical Reviews in Food Science and Nutrition,2021,61 (4): 649-665.
[28] YAZAWA S,SUETOM N,OKAMOTO K,et al. Content of capsaicinoids and capsaicinoid-like substances in fruit of pepper (Capsicum annuum L. ) hybrids made with ‘CH- 19 sweet’ as a parent
[J]. Journal of the Japanese Society for Horticultural Science,1989,58(3):601-607.
[29] LANG Y Q,KISAKA H,SUGIYAMA R,et al. Functional loss of pAMT results in biosynthesis of capsinoids,capsaici- noid analogs,in Capsicum annuum cv. CH-19 Sweet
[J]. The Plant Journal:for Cell and Molecular Biology,2009, 59(6):953-961.
[30] TANAKA Y,HOSOKAWA M,MIWA T,et al. Novel loss- of-function putative aminotransferase alleles cause biosyn- thesis of capsinoids,nonpungent capsaicinoid analogues,in mildly pungent chili peppers ( Capsicum chinense)
[ J]. Journal of Agricultural and Food Chemistry, 2010, 58(22):11762-11767.
[31] KOEDA S,SATO K,TOMI K,et al. Analysis of non-pun- gency,aroma,and origin of a Capsicum chinense cultivar from a Caribbean Island
[ J]. Journal of the Japanese Society for Horticultural Science,2014,83(3):244-251.
[32] PARK Y J,NISHIKAWA T,MINAMI M,et al. A low-pun- gency S3212 genotype of Capsicum frutescens caused by a mutation in the putative aminotransferase ( p-AMT ) gene
[J]. Molecular Genetics and Genomics, 2015, 290(6):2217-2224.
[33] JANG S,HAN K,JO Y D,et al. Substitution of a dysfunc- tional pAMT allele results in low-pungency but high levels of capsinoid in Capsicum chinense ‘habanero’
[J]. Plant Breeding and Biotechnology,2015,3(2):119-128.
[34] TANAKA Y,SONOYAMA T,MURAGA Y,et al. Multiple loss-of-function putative aminotransferase alleles contribute to low pungency and capsinoid biosynthesis in Capsicum chinense
[J]. Molecular Breeding,2015,35(6):142.
[35] TANAKA Y,FUKUTA S,KOEDA S,et al. Identification of a novel mutant pAMT allele responsible for low-pungency and capsinoid production in chili pepper:Accession ‘No. 4034 ’ ( Capsicum chinense )
[ J ]. The Horticulture Journal,2018,87(2):222-228.
[36] ARCE-RODRíGUEZ M L,OCHOA-ALEJO N. Biochemistry and molecular biology of capsaicinoid biosynthesis:Recent advances and perspectives
[J]. Plant Cell Reports,2019, 38(9):1017-1030.
[37] SANO K,UZAWA Y,KANESHIMA I,et al. Vanillin re- duction in the biosynthetic pathway of capsiate,a non-pun- gent component of Capsicum fruits, is catalyzed by cinnamyl alcohol dehydrogenase
[ J]. Scientific Reports, 2022,12:12384.
[38] JEONG H S,JANG S,HAN K,et al. Marker-assisted back- cross breeding for development of pepper varieties (Capsi- cum annuum) containing capsinoids
[J]. Molecular Breed- ing,2015,35(12):226.
[39] ZEWDIE Y,BOSLAND P W. Evaluation of genotype,environ- ment,and genotype-by-environment interaction for capsa- icinoids in Capsicum annuum L
[ J]. Euphytica, 2000, 111(3):185-190.
[40] STELLARI G M,MAZOUREK M,JAHN M M. Contrasting modes for loss of pungency between cultivated and wild species of Capsicum
[ J ]. Heredity, 2010, 104 ( 5 ): 460-471.
[41] HAN K,JANG S,LEE J H,et al. A MYB transcription fac- tor is a candidate to control pungency in Capsicum annu- um
[J]. Theoretical and Applied Genetics,2019,132(4): 1235-1246.
[42] YI S,LEE D G,BACK S,et al. Genetic mapping revealed that the Pun2 gene in Capsicum chacoense encodes a puta- tive aminotransferase
[ J ]. Frontiers in Plant Science, 2022,13:1039393.
[43] ARCE-RODRíGUEZ M L,OCHOA-ALEJO N. An R2R3- MYB transcription factor regulates capsaicinoid biosynthe- sis
[J]. Plant Physiology,2017,174(3):1359-1370.
[44] KOEDA S,SATO K,SAITO H,et al. Mutation in the puta- tive ketoacyl-ACP reductase CaKR1 induces loss of pun- gency in Capsicum
[J]. Theoretical and Applied Genetics, 2019,132(1):65-80.
[45] ALURU M R,MAZOUREK M,LANDRY L G,et al. Differ- ential expression of fatty acid synthase genes,Acl,Fat and Kas,in Capsicum fruit
[J]. Journal of Experimental Botany, 2003,54(388):1655-1664.
[46] KOEDA S,SATO K,TANAKA Y,et al. A Comt1 loss of function mutation is insufficient for loss of pungency in Capsicum
[J]. American Journal of Plant Sciences,2015, 6(8):1243-1255.
[47] DEL ROSARIO ABRAHAM-JUáREZ M,DEL CARMEN ROCHA-GRANADOS M, LóPEZ M G, et al. Virus- induced silencing of Comt,pAmt and Kas genes results in a reduction of capsaicinoid accumulation in chili pepper fruits
[J]. Planta,2008,227(3):681-695.
[48] YARNES S C,ASHRAFI H,REYES-CHIN-WO S,et al. I- dentification of QTLs for capsaicinoids, fruit quality, and plant architecture-related traits in an interspecific Capsicum RIL population
[J]. Genome,2013,56(1):61-74.
[49] BEN-CHAIM A,BOROVSKY Y,FALISE M,et al. QTL anal- ysis for capsaicinoid content in Capsicum
[J]. Theoretical and Applied Genetics,2006,113(8):1481-1490.
[50] BLUM E, MAZOUREK M, O ’ CONNELL M, et al. Molecular mapping of capsaicinoid biosynthesis genes and quantitative trait loci analysis for capsaicinoid content in Capsicum
[ J]. Theoretical and Applied Genetics,2003, 108(1):79-86.
[51] HAN K, LEE H Y, RO N Y, et al. QTL mapping and GWAS reveal candidate genes controlling capsaicinoid content in Capsicum
[ J]. Plant Biotechnology Journal, 2018,16(9):1546-1558.
[52] LEE J,PARK S J,HONG S C,et al. QTL mapping for cap- saicin and dihydrocapsaicin content in a population of Capsicum annuum ‘ NB1 ’ × Capsicum chinense ‘ Bhut Jolokia’
[J]. Plant Breeding,2016,135(3):376-383.
[53] NIMMAKAYALA P,ABBURI V L,SAMINATHAN T,et al. Genome-wide diversity and association mapping for cap- saicinoids and fruit weight in Capsicum annuum L[ J]. Scientific Reports,2016,6:38081.
[54] HILL T A,CHUNTHAWODTIPORN J,ASHRAFI H,et al. Regions underlying population structure and the genomics of organ size determination in Capsicum annuum
[J]. The Plant Genome,2017,10(3):10. 3835 / plantgenome2017. 03. 0026.
[55] SUN B M,ZHOU X,CHEN C M,et al. Coexpression net- work analysis reveals an MYB transcriptional activator in- volved in capsaicinoid biosynthesis in hot peppers
[ J]. Horticulture Research,2020,7:162.
[56] SUN B M,ZHU Z S,CHEN C J,et al. Jasmonate-inducible R2R3-MYB transcription factor regulates capsaicinoid bio- synthesis and stamen development in Capsicum
[ J ]. Journal of Agricultural and Food Chemistry, 2019, 67(39):10891-10903.
[57] WEN J F, LV J H, ZHAO K, et al. Ethylene-inducible AP2 / ERF transcription factor involved in the capsaicinoid biosynthesis in Capsicum
[J]. Frontiers in Plant Science, 2022,13:832669.
[58] 张威. 黄灯笼辣椒MYB4、MYB24 转录因子的克隆及功能鉴定
[D]. 海口:海南大学,2022.
[59] KIM J S,PARK M,LEE D J,et al. Characterization of pu- tative capsaicin synthase promoter activity
[ J]. Molecules and Cells,2009,28(4):331-339.
[60] ARCE-RODRíGUEZ M L, OCHOA-ALEJO N. Silencing AT3 gene reduces the expression of pAmt,BCAT,Kas,and Acl genes involved in capsaicinoid biosynthesis in chili pepper fruits
[ J ]. Biologia Plantarum, 2015, 59 ( 3 ): 477-484.
[61] TOHGE T,WENDENBURG R,ISHIHARA H,et al. Charac- terization of a recently evolved flavonol-phenylacyltrans- ferase gene provides signatures of natural light selection in Brassicaceae
[J]. Nature Communications,2016,7:12399.
[62] FRITZ C,PALACIOS-ROJAS N,FEIL R,et al. Regulation of secondary metabolism by the carbon-nitrogen status in tobacco:Nitrate inhibits large sectors of phenylpropanoid metabolism
[J]. The Plant Journal:for Cell and Molecular Biology,2006,46(4):533-548.
[63] SCHULZ E,TOHGE T,ZUTHER E,et al. Flavonoids are determinants of freezing tolerance and cold acclimation in Arabidopsis thaliana
[ J ]. Scientific Reports, 2016, 6:34027.
[64] CAMPOS-VARGAS R,NONOGAKI H,SUSLOW T,et al. Heat shock treatments delay the increase in wound- induced phenylalanine ammonia-lyase activity by altering its expression, not its induction in Romaine lettuce (Lactuca sativa) tissue
[J]. Physiologia Plantarum,2005, 123(1):82-91.
[65] TEKLEMARIAM T A,BLAKE T J. Phenylalanine ammonia- lyase-induced freezing tolerance in jack pine (Pinus banksi- ana) seedlings treated with low,ambient levels of ultraviolet- B radiation
[J]. Physiologia Plantarum, 2004, 122 ( 2 ): 244-253.
[66] SANCHEZ-BALLESTA M T, ZACARIAS L, GRANELL A,et al. Accumulation of PAL transcript and PAL activity as affected by heat-conditioning and low-temperature storage and its relation to chilling sensitivity in mandarin fruits
[ J]. Journal of Agricultural and Food Chemistry, 2000,48(7):2726-2731.
[67] LAFUENTE M T,SALA J M,ZACARIAS L. Active oxygen detoxifying enzymes and phenylalanine ammonia-lyase in the ethylene-induced chilling tolerance in citrus fruit
[J]. Journal of Agricultural and Food Chemistry, 2004, 52(11):3606-3611.
[68] CHEN J Y,WEN P F,KONG W F,et al. Effect of salicylic acid on phenylpropanoids and phenylalanine ammonia- lyase in harvested grape berries
[ J]. Postharvest Biology and Technology,2006,40(1):64-72.
[69] MURAKAMI K,IDO M,MASUDA M. Fruit pungency of ‘shishito’ pepper as affected by a dark interval in continuous fluorescent illumination with temperature alteration
[ J]. Shokubutsu Kankyo Kogaku,2006,18(4):284-289.
[70] ESTRADA B,POMAR F,DíAZ J,et al. Pungency level in fruits of the Padrón pepper with different water supply
[J]. Scientia Horticulturae,1999,81(4):385-396.
[71] CHABAANE Y,MARQUES ARCE C,GLAUSER G,et al. Altered capsaicin levels in domesticated chili pepper varie- ties affect the interaction between a generalist herbivore and its ectoparasitoid
[J]. Journal of Pest Science,2022, 95(2):735-747.
[72] BONAVENTURE G. Perception of insect feeding by plants
[J]. Plant Biology,2012,14(6):872-880.
[73] GUTIéRREZ-CARBAJAL M G,MONFORTE-GONZáLEZ M,DE L MIRANDA-HAM M,et al. Induction of capsaici- noid synthesis in Capsicum chinense cell cultures by sali- cylic acid or methyl jasmonate
[ J]. Biologia Plantarum, 2010,54(3):430-434.
[74] SUDHA G,RAVISHANKAR G. Influence of methyl jas- monate and salicylic acid in the enhancement of capsaicin production in cell suspension cultures of Capsicum frutescens Mill
[ J ]. Current Science, 2003, 85 ( 8 ): 1212-1217.
[75] OCHOA-ALEJO N, GóMEZ-PERALTA J E. Activity of enzymes involved in capsaicin biosynthesis in callus tissue and fruits of chili pepper ( Capsicum annuum L. )
[ J]. Journal of Plant Physiology,1993,141(2):147-152.
[76] SUN B M,CHEN C M,SONG J L,et al. The Capsicum MYB31 regulates capsaicinoid biosynthesis in the pepper pericarp
[ J]. Plant Physiology and Biochemistry,2022, 176:21-30.
[77] GIOVANNONI J,NGUYEN C,AMPOFO B,et al. The epig- enome and transcriptional dynamics of fruit ripening
[J]. Annual Review of Plant Biology,2017,68:61-84.
[78] JONES B,FRASSE P,OLMOS E,et al. Down-regulation of DR12,an auxin-response-factor homolog,in the tomato re- sults in a pleiotropic phenotype including dark green and blotchy ripening fruit
[J]. The Plant Journal:for Cell and Molecular Biology,2002,32(4):603-613.
[79] B?TTCHER C,KEYZERS R A,BOSS P K,et al. Seques- tration of auxin by the indole-3-acetic acid-amido synthetase GH3-1 in grape berry (Vitis vinifera L. ) and the proposed role of auxin conjugation during ripening
[J]. Journal of Ex- perimental Botany,2010,61(13):3615-3625.
[80] SUN L,YUAN B,ZHANG M,et al. Fruit-specific RNAi- mediated suppression of SlNCED1 increases both lycopene and β-carotene contents in tomato fruit
[J]. Journal of Ex- perimental Botany,2012,63(8):3097-3108.
本文详细论述了辣椒果实辣椒素类物质生物合成的分子遗传学研究进展,对该领域的研究进行了系统的梳理,对后续研究有很好的参考价值
该摘要清晰地勾勒出一篇高质量的综述性研究。选题具有重要的理论价值和应用前景,内容覆盖全面,结构逻辑清晰,表明该文献对该领域的研究进行了系统、深入的梳理和总结,对相关研究者具有很好的指导意义。